In cancer cells, autophagy can compensate for hypoxic conditions and nutrient starvation; on the other hand , activation of cell death via autophagy can kill tumor cells. As a result, there is a great interest in assays that can efficiently screen for activators and inhibitors in autophagy.
This application notes describes several new optimized assay kits specifically designed for autophagy evaluation by flow cytometry. Unlike current flow cytometry assays for autophagy, these assays can discriminate between cytosolic and autophagosome-associated LC3, thereby enabling robust, accurate evaluation of autophagy.
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