Kit Analyzes NFkB Translocation by Imaging Flow Cytometry

Amnis NFkB Translocation kit from EMD Millipore allows researchers to better study the nuclear translocation of NFkB, a transcription factor that plays a central role in regulating key mammalian cell processes, including proliferation, inflammation, immune and stress responses. The new kit uses imaging flow cytometry to obtain statistically significant quantitative assessment of NFкB translocation, as well as visual identification of the translocation at a single-cell level.

The optimized kit, which works with cultured cell lines and whole blood cells, conveniently contains directly-conjugated anti-Human NFкB monoclonal antibody, 7-AAD dye and required buffers. Using the dedicated Nuclear Localization Wizard in the IDEAS software, NFкB translocation can then be studied and quantified in an objective, statistically robust manner. The kit is designed for use with the Amnis ImageStreamX Mark II and the Amnis FlowSight imaging flow cytometers, systems which combine the quantitative power of flow cytometry with the spatial information provided by microscopy.

EMD Millipore, www.emdmillipore.com/amnis