Quantitation of Apoptosis by Nuclear Fragmentation

In this report, apoptosis was induced in HL60 cells with daunorubicin, a DNA-intercalating agent which inhibits DNA and RNA synthesis and is used as a treatment for acute myeloid leukemia (AML). HL60 cells were exposed to a time course of daunorubucin treatment, and the apoptotic rate was measured directly from nuclear images obtained with the ImageStream system.