Established Cell Lines

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Primary Human Umbilical Vein Endothelial Cells

ATCC (American Type Culture Collection) offers human umbilical vein endothelial cells (HUVEC) as part of its Primary Cell Solutions for cardiovascular research. These primary cells are derived from either single donors or from a pooled population of unique donors.

Human Retinal Pigment Epithelial Cells and RtEGM Growth Medium

Lonza offers cryopreserved Clonetics Human Retinal Pigment Epithelial (hRPE) Cells and optimized RtEGM Growth Medium BulletKit for ophthalmologic research including age-related macular degeneration, retinistis pigmentosa, and gene expression profiles of RPE.

Normal Human Prostate Epithelial Cells

The newest addition to the ATCC Primary Cell Solutions collection are Normal Human Prostate Epithelial Cells derived from normal prostate.

Pancreatic Alpha Cell Line

The pancreatic alpha cell line, Alpha TC1 clone 6 (ATCC CRL-2934), is a useful model in diabetes research for studying many aspects of islet biology, including glucagon biosynthesis and cytokine sensitivity.

Human Placenta Derived Basement Membrane Extract

Trevigen has developed a Basement Membrane Extract (BME) derived from normal human placenta, avoiding the presence of both tumor-derived and mouse-specific proteins in the human cell microenvironment.

Human Natural Killer Cells

Poietics cryopreserved, ready-to-use primary cells for immunology research are isolated from normal human peripheral blood using positive or negative immunomagnetic selection for the CD56 antigen. These cells are tested to be 90% pure for CD56+ via flow cytometry, and 95% viable after thaw.

Using Mammalian Competent Cells For Evaluation Of IRES Sequences

Expression of multiple recombinant proteins in mammalian cells from a single mRNA is technically more difficult than expression in bacterial cells due to the different nature by which translational initiation occurs. In bacteria, a single mRNA molecule may encode multiple open reading frames with every gene product initiating from a ribosome-binding site preceding the start codon.

Stem Cell Monitoring Kit

The STEMPRO EZChek Multiplex PCR Kit allows scientists to monitor hESCs (human embryonic stem cells) with one reaction, using fewer sample cells than ever before. Four early differentiation markers can be analyzed using a small sample and a single-tube reaction.

Neonatal Rat Cardiac Myocytes

Clonetics Neonatal Rat Cardiac Myocytes are available for use in cardiovascular and toxicological research. These cryopreserved cells offer functional, beating cardiac myocytes upon thaw, at the user’s convenience.

Engineered Stem Cell Line

This engineered stem cell line allows scientists to monitor the pluripotency of human embryonic stem cells (hESCs) without sacrificing those cells.

Research Exchange

Bringing the Cell Image into Focus

Nov 2

Improvements in transmission electron microscope (TEM) technology increase the power of this imaging tool for the study of cell biology.

Finding a Cure for Spinal Cord Injury with On-Demand LIMS

Oct 25

The Miami Project to Cure Paralysis finds an on-demand laboratory information management system (LIMS) helps to accelerate discovery in its HCS projects.

Saving Cells: Image Processing for Improved Viability, Part II: Iterative Deconvolution

Oct 25

3D light microscopy and deconvolution provide a means to investigate 3D structure, providing near-confocal quality images without the temporal requirements or potentially damaging phototoxicity associated with other 3D imaging technologies. This article is Part II in a series regarding viability, resolution improvement, and measurement in fluorescence imaging. Part I focused on spectral unmixing.

Saving Cells: Image Processing for Improved Viability

Sep 22

This article is Part I of a two-part series regarding viability, resolution improvement, and measurement in fluorescence imaging. Part II will focus on deconvolution.

HP Scalable Network Storage Systems for Life Sciences

Sep 13

Life sciences research today is advancing exponentially, each step bringing us closer to the realization of truly personalized medicine–preventive care and treatments designed specifically for each individual. In the near future, PCPGM healthcare researchers expect to be able to use predictive genetic testing to create custom treatment plans for individuals and deliver dramatic improvements over today’s one-size-fits-all approach. But research capabilities are only part of the equation; current storage and operating capacities must also evolve to accommodate ever-expanding amounts of data before the goal of personalized medicine can be realized.

Step up to the MIQE

Mar 30

Over the years, polymerase chain reaction (PCR) has evolved into a readily automated, high throughput quantitative technology. Real-time quantitative PCR (qPCR) has become the industry standard for the detection and quantification of nucleic acids for multiple application, including quantification of RNA levels. But a lack of consensus among researchers on how to best perform and interpret qPCR experiments presents a major hurdle for advancement of the technology. This problem is exacerbated by insufficient experimental detail in published work, which impedes the ability of others to accurately evaluate or replicate reported results.

Fast Optimization of a Multiplex Influenza Identification Panel Using a Thermal Gradient

Mar 30

The year 2009 was marked by the emergence of a novel influenza A (H1N1) virus that infects humans. There is a need to identify the different strains of influenza virus for purposes of monitoring the H1N1 strain pandemic and for other epidemiological and scientific purposes.

Advantages of Monolithic Laser Combiner Technology in Confocal Microscopy Systems

Jan 6

Fluorescence microscopy techniques require a reliable light source at the desired wavelength or wavelengths, with minimal downtime for maintenance and alignment. Lasers are a popular light source, although the alignment and upkeep of laser combiners is a time-consuming prospect for many users.

Using the Tecan Genesis Workstation to Automate a Cytometric Bead Array (CBA) Immunoassay

Mar 11

The poster describe the process involved in automating a Cytometric Bead Array (CBA) immunoassay developed to measure relative concentrations of serum antibodies against Tetanus (TT), Sperm Whale Myoglobin (SWM) and Keyhole Limpet Hemocyanin (KLH) in KLH-immunized volunteers.

Ensuring Quality in Assays Performed with Automated Liquid Handlers

Feb 2

The focus of this presentation is to highlight the need of ensuring quality in important assays performed with automated liquid handlers. Nearly all assays performed within a laboratory are volume-dependent. In turn, all concentrations of biological and chemical components in these assays, as well as the associated dilution protocols, are volume-dependent. Because analyte concentration is volume-dependent, an assay’s results might be falsely interpreted if liquid handler variability and inaccuracies are unknown or if the system(s) go unchecked for a long period.

Inkjet System for Protein Crystallography

Feb 1

X-ray crystallography is used routinely by scientists to obtain the three dimensional structure of a biological molecule of interest.Such information can be used to determine how a pharmaceutical interacts with a protein target and what changes might improve functionality. However, the crystallization of macromolecules still remains a serious hindrance in structural determination despite impressive advances in screening methods and technologies.

Attention Deficit & Hyperactivity in a Drosophila Memory Mutant

Attention Deficit & Hyperactivity in a Drosophila Memory Mutant

Nov 9 2009

Action selection is modulated by external stimuli either directly or via memory retrieval. In a constantly changing environment, animals have evolved attention-like processes to effectively filter the incoming sensory stream. These attention-like processes, in turn, are modulated by memory. The neurobiological nature of how attention, action selection and memory are inter-connected is unknown. We describe here new phenotypes of the memory mutant radish in the fruit fly Drosophila.

Comparison of exhaled carbon monoxide levels among commuters and roadside vendors in an urban and a suburban population in Pakistan.

1 hour ago

Carbon monoxide (CO) is one of the six criteria air pollutants related to urbanization and has a wide range of health effects. The study measured and compared the exhaled CO levels among commuters and roadside vendors in potentially heavy and low traffic volume...

Convergence of the 26S proteasome and the REVOLUTA pathways in regulating inflorescence and floral meristem functions in Arabidopsis.

3 hours ago

The 26S proteasome is a large multisubunit proteolytic complex, regulating growth and development in eukaryotes by selective removal of short-lived regulatory proteins. Here, it is shown that the 26S proteasome and the transcription factor gene REVOLUTA (REV) act together in...

Haem oxygenase delays programmed cell death in wheat aleurone layers by modulation of hydrogen peroxide metabolism.

3 hours ago

Haem oxygenase-1 (HO-1) confers protection against a variety of oxidant-induced cell and tissue injury in animals and plants. In this report, it is confirmed that programmed cell death (PCD) in wheat aleurone layers is stimulated by GA and prevented by ABA. Meanwhile, HO...

Is LEAFY a useful marker gene for the flower-inflorescence boundary in the Euphorbia cyathium?

3 hours ago

The flower-like reproductive structure of Euphorbia s.l. (Euphorbiaceae) is widely believed to have evolved from an inflorescence, and is therefore interpreted as a special type of pseudanthium, termed a cyathium. However, fuzzy morphological boundaries between...

Prokariotic Cell Collection in Denmark

Nov 6 2009

I would like to know about a prokariotic cell collection in Denmark. Is there a cell bank in this country? I need a Lactobacillus strain for a fermentation assay and this information about the bank is very helpful for me.

Request for Entries

Oct 16 2009

Ask the Experts is your chance to get the answers to questions on applications, materials, methods, processes, and technologies. Email you question to bst_web@advantagemedia.com, and the editors of Bioscience Technology will find an appropriate expert to answer it. Watch this space in the future to see the questions your colleagues are posting.

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Video:

Viewing SureFocus Slides

Jun 11

A demonstration of SureFocus Microscope Slides in the review of AFB Smears. SureFocus Slides are a patent-pending breakthrough in tuberculosis detection, as their fluorescent staining circle remains visible during review, Fluorescence Microscopy.

Podcasts:

Allen Institute for Brain Research

Allen Institute for Brain Research

Oct 14 2009

Discussed in this interview are both the mouse brain project and the human cortex project with an emphasis on the importance of these projects to neuroscience research.

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