The EnduraTEC ITEMS (Intelligent Tissue Engineering via Mechanical Stimulation) BioReactor is a scalable biodynamic environmental system that provides a complete closed-loop nutrient-flow subsystem capable of introducing mechanical stresses and strains to a tissue engineered construct during its growth cycle. In addition to mechanical stimulation, the researcher can query the tissue construct to evaluate its mechanical condition. Using WinTest® software, the tissue's complex modulus can be estimated. This procedure allows adjustment of the stimulation profile to best meet the desired growth plan for the chosen tissue engineered construct. Researchers can seed constructs and apply pressure/flow waveforms of varying shapes and frequencies to stimulate cell proliferation and matrix deposition leading to constructs with properties suitable for use in humans. The waveforms are generated using a computer-controlled dynamic pumping system and allow experimentation with different pressure/flow conditions. The bioreactor is available in a single chamber design or in a six chamber design for development of tissue engineered blood vessels, grafts, or valves. Variations of the system are available for direct stimulation of other tissues, including bone and ligaments. For cartilage applications, the multi-station BioReactor has the capability to directly stimulate six scaffolds simultaneously with both axial and shear loading.