ORANGE COUNTY, CALIF. (December 15, 2023) – In a
paper published in the November/December 2010 issue of
Biotechnology Progress (Vol. 26, No. 6) the authors use
the large amounts of data generated by Beckman Coulter’s
Multisizer COULTER COUNTER to infer significant and detailed
information about cells infected with viruses. (Originally
published online, Sept. 23, 2010 on the Biotechnology
Progress "Early View" page by the American Institute of
Chemical Engineers).
The paper, entitled "Getting More from Cell Size Distributions:
Establishing More Accurate Biovolumes by Estimating Viable Cell
Populations," was written by Professor Marc Aucoin and his
colleagues at the University of Waterloo in Ontario, Canada.
The authors used sophisticated regression analyses to study the
size distributions of insect cells before and after they were
infected with viral vectors. The analyses helped the authors
identify two regions of cell distribution that were highly
correlated with cell viability at one, two and three days,
post-infection.
"Many investigators have found the rich data produced by
instruments in the Multisizer series invaluable for addressing
questions related to cell size distribution," said Matthew Rhyner,
Ph.D., product manager with Beckman Coulter Particle
Characterization. "As this work demonstrates, the detailed
analysis enabled by the Multisizer shows the usefulness of studying
the entire cell size distribution as opposed to focusing on mean
cell diameters."
"While the viability study is interesting and informative,
it’s likely only the tip of the iceberg in terms of what
information can be gained from analyzing full cell size
distributions," said Professor Aucoin, corresponding author of the
study. "There’s a tremendous amount of data contained
in each distribution, above and beyond simple mean diameters.
Our group hopes to conduct similar studies in the future and see
just how far we can push the approach."
Elsa Burgess, director of Beckman Coulter Particle
Characterization said, "We believe it’s important to use
input from customers such as those at the University of Waterloo to
improve and add to the data producing capabilities of the
Multisizer, and the work of Professor Aucoin and his group clearly
shows how scientists, and research in general, benefits from
improvements that are made based on these interactions."
The Beckman Coulter Particle Characterization Business Center is
based in Miami, Florida. The group develops, manufactures and
markets products that are used to study, analyze and quantify
particles of any type and serves customers from cell biologists to
pharmaceutical companies to cement manufacturers. Serving
particle customers since 1960, the group specializes in the Coulter
Principle, laser diffraction, dynamic light scattering, zeta
potential and BET analysis to understand all aspects of particulate
samples. For more information, visit www.coultercounter.com.
About Beckman Coulter
Beckman Coulter, Inc., based in Orange County, California,
develops, manufactures and markets products that simplify, automate
and innovate complex biomedical tests. This year marks the
company’s 75th anniversary of advancing healthcare. More than
200,000 Beckman Coulter systems operate in laboratories around the
world, supplying critical information for improving patient health
and reducing the cost of care. Recurring revenue, consisting of
consumable supplies (including reagent test kits), service and
operating-type lease payments, represents about 80 percent of the
company's 2009 revenue of $3.3 billion. For more information, visit
www.beckmancoulter.com or
www.beckmancoulter.com/75years.
Beckman Coulter, Coulter Counter and the stylized logo are
registered trademarks of Beckman Coulter, Inc.
Multisizer is a registered trademark of Beckman Coulter, Inc.
SOURCE