The Hybridizer ISH instrument (DakoCytomation) complements the Autostainer Plus and other staining systems, and allows for semi-automation of ISH by eliminating steps in the time-intensive manual procedure. In situ hybridization (ISH) techniques localize specific nucleic acid sequences within patient specimens. Development of fluorescent in situ hybridization (FISH) and chromogenic in situ hybridization (CISH) has made molecular testing attractive for routine applications including gene expression studies. The Hybridizer is a hands-free denaturation and hybridization instrument for slide-based FISH and CISH on tissue and cell samples. The programmable system, featuring short heating and cooling times as well as a humidity control system, ensures consistent results for a range of probe applications.