AVIA Biosystems’ Model 2304 automated protein denaturation system automates protein stability determinations using chemical denaturation. Developed to facilitate the formulation of biologics in a safe and stable form, the system uses intrinsic or extrinsic fluorescence to monitor the conformational changes associated with protein unfolding (denaturation) and automatically generates complete protein stability curves. Up to 96 different formulations, process conditions or individual protein constructs can be evaluated for relative stability, and an individual stability curve generated for each. Sample preparation, data collection, data analysis and stability report-generation are all automated. The free energy (ΔG) of protein unfolding is automatically calculated for each formulation condition or each individual protein construct. Chemical denaturation provides reliable thermodynamic values for protein stability and allows solution conditions such as buffer composition, buffer strength, pH, ionic strength, excipient composition, excipient concentration and protein concentration to be effectively assessed and optimized.

AVIA Biosystems, 413-658-5426, www.aviabiosystems.com