The NUNC LiveCell Array, a microscope slide-based high content analysis (HCA) tool, enables multi-parametric imaging-based assays on thousands of intact individual cells, including non-adhering blood and bone marrow cells. Cells can be observed in their own identified location, tracking individual, real-time responses to intervention. Multiple functional assays can be performed on a living cell, followed by post-fixation studies on the same cell to maximize usage of cell samples. The disposable slides are offered with an embedded, transparent array of pico-wells ranging from 15 to 20, 100 or 250 microns in diameter. The design facilitates the flow of reactants, reagents and media solutions, and enables perfusion of the cells in the array wells. Individual cells settle into the pico-wells by means of gravity, and are not displaced by rinsing or staining. Deployment of available iImaging and data analysis software allows scientists to designate an address to each cell. In addition to the availability of a stand-alone slide, NUNC LiveCell Array is also supplied in kits that include reagents, buffers and protocols for assays involving apoptosis or CD3, CD4 and CD8 cell surface markers.