Size-Exclusion Chromatography for Purification of Biomolecules

Dec 2

Size-exclusion chromatography (SEC) is a popular method to separate biomolecules based on their size. Primarily, it is applied to the separation of biopolymers such as proteins and nucleic acids, i.e. water-soluble polymers.

Improving Separation During Electrophoresis

Dec 2

SeparateIT gels represent a novel gel matrix for DNA electrophoresis. Gel polymers are arranged in a conceptually different way, in accordance with a new theoretical model of gel electrophoresis.

Improving Quality of ELISA

Dec 2

Using ready-to-use ELISA kits from manufacturers is easy and convenient. Sometimes however, home-made ELISA is required because there is no kit available with the right antibodies or the characteristics of the available kits such as their limits of detection are not appropriate.

Nucleic Acid Decontamination with the ExitusPlus Technology

Dec 2

Using new methods that detect extremely low levels of DNA molecules, researchers investigated the molecular mechanism of action of various commercially available DNA decontamination reagents. They found that when using high concentrations of DNA and short incubation times, none of the conventional reagents destroyed DNA molecules efficiently, despite their corrosive or even toxic properties.

Attention Deficit & Hyperactivity in a Drosophila Memory Mutant

Nov 9

Action selection is modulated by external stimuli either directly or via memory retrieval. In a constantly changing environment, animals have evolved attention-like processes to effectively filter the incoming sensory stream. These attention-like processes, in turn, are modulated by memory. The neurobiological nature of how attention, action selection and memory are inter-connected is unknown. We describe here new phenotypes of the memory mutant radish in the fruit fly Drosophila.

Mechanisms of plasticity in simple taxis behaviors in Drosophila

Nov 8

Like the proverbial moth drawn to the candle flame, the fruit fly Drosophila also stereotypically approaches light sources. This positive phototaxis is the archetypal example of hard-wired input-output behaviors.

Live cell imaging of mutant and wild-type GABA-A receptor trafficking using a novel reporter protein

Nov 8

Efficient signaling in the brain requires precise regulation and targeting of cell surface ion-channels. Mutations in these channels associated with inherited diseases can cause improper targeting and reduced surface expression.

ADP-Glo™: An Ideal Approach to Monitor the Activity of Protein Kinases and Beyond

Oct 16

Because of its versatility (alltypesofsubstrates), robustness (Z’>0.8), rapidperformance, and its ease of use, the luminescence based Kinase Glo® assay platform has gained wide acceptance in many drug screening programs for protein kinase inhibitors.

A role for Chk2 in DNA damage induced mitotic delays in human colorectal cancer cells.

34 minutes ago

Progression into mitosis in the presence of DNA damage leads to spindle checkpoint (SAC) dependent mitotic delays and cytokinesis failure. In Drosophila embryos, DNA damage does not delay mitotic entry but triggers Checkpoint kinase-2 (Chk2) kinase dependent...

An ex vivo swine tracheal organ culture for the study of influenza infection.

1 hour ago

Please cite this paper as: Nunes et al. (2009) An ex vivo swine tracheal organ culture for the study of influenza infection. Influenza and Other Respiratory Viruses 4(1), 7-15.Background The threat posed by swine influenza viruses with potential to transmit from...

The effect of age on the pathogenesis of a highly pathogenic avian influenza (HPAI) H5N1 virus in Pekin ducks (Anas platyrhynchos) infected experimentally.

1 hour ago

Please cite this paper as: Löndt BZ et al. (2009). The effect of age on the pathogenesis of a highly pathogenic avian influenza (HPAI) H5N1 virus in Pekin (Anas platyrhynchos) ducks infected experimentally. Influenza and Other Respiratory Viruses 4(1), 17-25....

Identifying viral infections in vaccinated Chronic Obstructive Pulmonary Disease (COPD) patients using clinical features and inflammatory markers.

1 hour ago

Please cite this paper as: Hutchinson et al. (2009) Identifying viral infections in vaccinated Chronic Obstructive Pulmonary Disease (COPD) patients using clinical features and inflammatory markers. Influenza and Other Respiratory Viruses 4(1), 33-39.Background...

Prokariotic Cell Collection in Denmark

Nov 6

I would like to know about a prokariotic cell collection in Denmark. Is there a cell bank in this country? I need a Lactobacillus strain for a fermentation assay and this information about the bank is very helpful for me.

Request for Entries

Oct 16

Ask the Experts is your chance to get the answers to questions on applications, materials, methods, processes, and technologies. Email you question to bst_web@advantagemedia.com, and the editors of Bioscience Technology will find an appropriate expert to answer it. Watch this space in the future to see the questions your colleagues are posting.