Thermo Fisher Scientific and Intrinsic Bioprobes Announce Alliance in Mass Spectrometric Immunoassay (MSIA) Workflow Development

Featured In: Supplier News

Wednesday, July 21, 2024

Loading...

Thermo Fisher Scientific announced an alliance to co-develop and market a mass spectrometry solution that will enable researchers to perform quantitative, high-throughput, high-resolution protein biomarker assays. The solution couples the specificity of immunoenrichment with the sensitivity and quantitative capability of mass spectrometry to provide researchers with a more complete, higher-resolution view of the proteome. Initially, via an exclusive reseller agreement, the solution will be applied to the detection of human parathyroid hormone (PTH) and its variants, which may be useful as biomarkers for bone diseases and diseases of the endocrine system.

As part of the arrangement, Thermo Fisher Scientific will integrate IBI's proprietary immunoenrichment technology with Thermo Scientific automated sample handling and mass spectrometer systems. Scientists at the Biomarkers Initiatives in Mass Spectrometry (BRIMS) Center, a Thermo Fisher Scientific center of excellence, will collaborate with IBI to develop a mass spectrometric immunoassay (MSIA) workflow for research laboratories. Thermo Fisher Scientific will market, distribute and support the solution and the workflow.

"IBI's approach to biomarker analysis relies on our proprietary MSIA technologies for high-throughput, top-down protein assays," said Dobrin Nedelkov, Ph.D., chief executive officer, Intrinsic Bioprobes. "Our protein assays have been used extensively for biomarker analysis and validation, resulting in the discovery of several novel protein variants with important implications. The combination of our MSIA technology and Thermo Fisher Scientific's robust, automated sample handling, mass spectrometry systems and expertise in clinical research workflows, as well as its global distribution and support, will provide clinical researchers with a turnkey solution."

IBI's proprietary immunoenrichment technology is based on its patented pipette tip that integrates a high-throughput, high-binding-capacity microcolumn activated with antibodies. This technology addresses one of the key challenges of biomarker discovery and validation, the isolation and analysis of very low-abundance proteins such as PTH in complex biological matrices. Conventional approaches involving depletion of interfering high-abundance proteins are time-consuming and introduce analytical variability. Compared to other immunoenrichment approaches, IBI's approach offers more effective capture of low-abundance proteins.

A key challenge in proteomics research is the ability to differentiate between, and accurately quantitate, intact proteins and their variants. Traditional enzyme-linked immunosorbent assays (ELISA) are limited by the inability of the antibodies to discriminate between all variants and quantify their abundance. A solution is to use the specificity of mass spectrometry to identify and quantitate microheterogeniety. However, many researchers have been stymied by the lack of an integrated, high-throughput mass spectrometry workflow that is robust and reproducible from laboratory-to-laboratory.

"We are extremely enthusiastic about incorporating IBI's proprietary technology into comprehensive workflows that couple immunoenrichment to the power of high-resolution, quantitative, mass spectrometry," said Mary Lopez, Ph.D., director, Thermo Fisher Scientific's BRIMS Center. "Over the last year, we have worked closely with IBI to develop a reliable, quantitative assay for parathyroid hormone and its variants. This work, recently published in the Journal of Clinical Chemistry, demonstrates a workflow that enables detection and quantification of very-low-abundance intact PTH, and more importantly, several novel PTH protein variants that cannot be detected using conventional assays."

Thermo Fisher Scientific Inc., 800-532-4742, www.thermoscientific.com/ms
Booth #503, 2415

Join the Discussion
Rate Article: Average 0 out of 5
register or log in to comment on this article!

0 Comments

Add Comment

Text Only 2000 character limit

Page 1 of 1

Research Exchange

Optical Imaging in Stem Cell Research

Jun 1

Developing sensitive, non-invasive technologies to monitor engraftment in vivo is essential to accelerate the clinical implementation of cell therapies.

Standing Up in a Court of Law

Jun 1

Private forensic testing laboratory ensures data integrity with advanced calibration systems.

Simultaneous Measurement of Multiple Signaling Pathways in Human Leukemias Using Flow Cytometry

May 24

Next generation assays will need to be robust and standardized in order to make the transition from a research procedure to a routine clinical assay. Flow cytometry provides a unique and sensitive method to accomplish these requirements.

Automated Forensic DNA Methods: Relieving the Pain of Validation

Apr 21

Automation can increase a forensics lab's sample processing capacity, but it can also add to the complexity of system verification and validation.

Step up to the MIQE

Mar 30

Over the years, polymerase chain reaction (PCR) has evolved into a readily automated, high throughput quantitative technology. Real-time quantitative PCR (qPCR) has become the industry standard for the detection and quantification of nucleic acids for multiple application, including quantification of RNA levels. But a lack of consensus among researchers on how to best perform and interpret qPCR experiments presents a major hurdle for advancement of the technology. This problem is exacerbated by insufficient experimental detail in published work, which impedes the ability of others to accurately evaluate or replicate reported results.

Fast Optimization of a Multiplex Influenza Identification Panel Using a Thermal Gradient

Mar 30

The year 2009 was marked by the emergence of a novel influenza A (H1N1) virus that infects humans. There is a need to identify the different strains of influenza virus for purposes of monitoring the H1N1 strain pandemic and for other epidemiological and scientific purposes.

Advantages of Monolithic Laser Combiner Technology in Confocal Microscopy Systems

Jan 6

Fluorescence microscopy techniques require a reliable light source at the desired wavelength or wavelengths, with minimal downtime for maintenance and alignment. Lasers are a popular light source, although the alignment and upkeep of laser combiners is a time-consuming prospect for many users.

Size-Exclusion Chromatography for Purification of Biomolecules

Dec 2 2009

Size-exclusion chromatography (SEC) is a popular method to separate biomolecules based on their size. Primarily, it is applied to the separation of biopolymers such as proteins and nucleic acids, i.e. water-soluble polymers.

Using the Tecan Genesis Workstation to Automate a Cytometric Bead Array (CBA) Immunoassay

Mar 11

The poster describe the process involved in automating a Cytometric Bead Array (CBA) immunoassay developed to measure relative concentrations of serum antibodies against Tetanus (TT), Sperm Whale Myoglobin (SWM) and Keyhole Limpet Hemocyanin (KLH) in KLH-immunized volunteers.

Ensuring Quality in Assays Performed with Automated Liquid Handlers

Feb 2

The focus of this presentation is to highlight the need of ensuring quality in important assays performed with automated liquid handlers. Nearly all assays performed within a laboratory are volume-dependent. In turn, all concentrations of biological and chemical components in these assays, as well as the associated dilution protocols, are volume-dependent. Because analyte concentration is volume-dependent, an assay’s results might be falsely interpreted if liquid handler variability and inaccuracies are unknown or if the system(s) go unchecked for a long period.

Inkjet System for Protein Crystallography

Feb 1

X-ray crystallography is used routinely by scientists to obtain the three dimensional structure of a biological molecule of interest.Such information can be used to determine how a pharmaceutical interacts with a protein target and what changes might improve functionality. However, the crystallization of macromolecules still remains a serious hindrance in structural determination despite impressive advances in screening methods and technologies.

Attention Deficit & Hyperactivity in a Drosophila Memory Mutant

Attention Deficit & Hyperactivity in a Drosophila Memory Mutant

Nov 9 2009

Action selection is modulated by external stimuli either directly or via memory retrieval. In a constantly changing environment, animals have evolved attention-like processes to effectively filter the incoming sensory stream. These attention-like processes, in turn, are modulated by memory. The neurobiological nature of how attention, action selection and memory are inter-connected is unknown. We describe here new phenotypes of the memory mutant radish in the fruit fly Drosophila.

Relation of Atherosclerotic Cardiovascular Events to Progression of Aortic Stenosis in Older Men.

1 hour ago

BACKGROUND: Aortic stenosis (AS) is now considered to be an active disease like atherosclerosis. However, therapies such as statins have not shown benefit in AS patients when studied prospectively. HYPOTHESIS: We tested the hypothesis that if AS was indeed...

Native Valve Brucella Endocarditis.

1 hour ago

OBJECTIVE: Brucellosis is frequently seen in Mediterranean and Middle East countries, including Turkey. We report the medical and surgical management of 31 cases of native endocarditis. MATERIAL AND METHOD: Thirty-one patients were admitted to our clinic with...

Characterization of a novel novobiocin analogue as a putative C-terminal inhibitor of heat shock protein 90 in prostate cancer cells.

17 hours ago

PURPOSE: Hsp90 is important in the folding, maturation and stabilization of pro-tumorigenic client proteins and represents a viable drug target for the design of chemotherapies. Previously, we reported the development of novobiocin analogues designed to inhibit...

Long term increased expression of the short form 1b prolactin receptor in PC-3 human prostate cancer cells decreases cell growth and migration, and causes multiple changes in ...

17 hours ago

BACKGROUND: We have shown that treatment of human prostate cancer cells with the selective prolactin (PRL) receptor modulator, S179D PRL, inhibits growth in vitro, and the initiation and growth of xenografts in vivo. S179D PRL treatment also upregulates...

Prokariotic Cell Collection in Denmark

Nov 6 2009

I would like to know about a prokariotic cell collection in Denmark. Is there a cell bank in this country? I need a Lactobacillus strain for a fermentation assay and this information about the bank is very helpful for me.

Request for Entries

Oct 16 2009

Ask the Experts is your chance to get the answers to questions on applications, materials, methods, processes, and technologies. Email you question to bst_web@advantagemedia.com, and the editors of Bioscience Technology will find an appropriate expert to answer it. Watch this space in the future to see the questions your colleagues are posting.

STAY INFORMED: SUBSCRIBE TO

Magazine and E-mail Newsletters

Loading...
E-mail:   

MULTIMEDIA

Video:

Viewing SureFocus Slides

Jun 11

A demonstration of SureFocus Microscope Slides in the review of AFB Smears. SureFocus Slides are a patent-pending breakthrough in tuberculosis detection, as their fluorescent staining circle remains visible during review, Fluorescence Microscopy.

Podcasts:

Allen Institute for Brain Research

Allen Institute for Brain Research

Oct 14 2009

Discussed in this interview are both the mouse brain project and the human cortex project with an emphasis on the importance of these projects to neuroscience research.

Information: