ORANGE COUNTY, Calif. (May 11, 2024) The Cell Lab
Signaling Cytometry (Cell Lab SC) line of reagents from Beckman
Coulter, Inc., offers a comprehensive set of antibodies for the
detection of the phosphorylation state of signaling proteins in
cells via flow cytometry. Performing cell signaling
experiments via flow enables the user to generate precise, specific
and quantitative measurements of the signaling response in
individual cellular subpopulations.
The reagents, which in the initial offering are conjugated to
either Alexa Fluor* 488 or Alexa Fluor 647, are released in
conjunction with a new method that allows analysis of samples in
whole blood. Analyzing samples in whole blood minimizes
artifacts and variability introduced through sample manipulation
and helps preserve the native biology of the cell. The method
has also shown excellent performance when used with bone marrow,
cell culture, and purified cell populations.
Antibodies in the Cell Lab SC line can be used in combination
with extracellular markers to study signaling patterns in specific
cellular subpopulations. Products are available for the study
of apoptosis, chromatin regulation, cytoskeletal signaling, DNA
damage, lymphocyte signaling, MAP kinase signaling, protein
folding, translation control and other phosphorylation events.
"Using flow cytometry to get quantitative, cell-type specific
data enables researchers to interrogate signaling pathways in ways
previously unattainable by Western blotting," said Brad Calvin,
vice president of Beckman Coulter's Flow Cytometry Business
Center. "The combined whole-blood application, which
incorporates robust cell preparation and high affinity antibodies,
creates the potential to transform our understanding of cell
signaling’s role in disease, while also providing the
pharmaceutical industry with the potential for improved
characterization of target compound function and effect."
About Beckman Coulter
Beckman Coulter, Inc., based in Orange County, California,
develops, manufactures and markets products that simplify,
automate and innovate complex biomedical tests. More than
200,000 Beckman Coulter systems operate in laboratories around the
world, supplying critical information for improving patient health
and reducing the cost of care. Recurring revenue, consisting of
consumable supplies (including reagent test kits), service and
operating-type lease payments, represent about 80 percent of the
company's 2009 revenue of $3.3 billion. For more information, visit
http://www.beckmancoulter.com/.
Photo caption: Cell Lab SC reagents from Beckman Coulter, Inc.
detect the phosphorylation state of signaling proteins in cells
directly from whole blood via flow cytometry
*Alexa Fluor is a trademark of Molecular Probes (Invitrogen
Corp.).
Beckman Coulter and the stylized logo are registered trademarks of
Beckman Coulter, Inc.
Cell Lab and Signaling Cytometry are trademarks of Beckman Coulter,
Inc.
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