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Calibration Free Analysis to Measure the Concentration of Active Proteins
Nov 23
An SPR-based method, Calibration Free Concentration Analysis can be used to accurately determine the concentration of active protein in a sample, relating to the specific binding activity of the protein, and without the need for a standard.
Advances in EMCCD Technology: Making Imaging Less Arbitrary
Nov 16
Recent advances in EMCCD technology have solved the problem of non-standardized measurement units by using the photoelectron to standardize imaging experiments.
10 Tips for Successful Sample Concentration and Buffer Exchange
Nov 6
Centrifugal devices with ultrafiltration (UF) membrane can solve common problems researchers face when working with proteins.
Meeting the Challenges of Long-term Time-lapse Imaging
Oct 26
Recent technological developments in the sequence capture of cellular events through a light microscope can be combined to image multiple independent experiments automatically, with many advantages.
more APPLICATION NOTES >>
Size-Exclusion Chromatography for Purification of Biomolecules
Dec 2
Size-exclusion chromatography (SEC) is a popular method to separate biomolecules based on their size. Primarily, it is applied to the separation of biopolymers such as proteins and nucleic acids, i.e. water-soluble polymers.
Improving Separation During Electrophoresis
SeparateIT gels represent a novel gel matrix for DNA electrophoresis. Gel polymers are arranged in a conceptually different way, in accordance with a new theoretical model of gel electrophoresis.
Improving Quality of ELISA
Using ready-to-use ELISA kits from manufacturers is easy and convenient. Sometimes however, home-made ELISA is required because there is no kit available with the right antibodies or the characteristics of the available kits such as their limits of detection are not appropriate.
Nucleic Acid Decontamination with the ExitusPlus Technology
Using new methods that detect extremely low levels of DNA molecules, researchers investigated the molecular mechanism of action of various commercially available DNA decontamination reagents. They found that when using high concentrations of DNA and short incubation times, none of the conventional reagents destroyed DNA molecules efficiently, despite their corrosive or even toxic properties.
more WHITE PAPERS >>
Attention Deficit & Hyperactivity in a Drosophila Memory Mutant
Nov 9
Action selection is modulated by external stimuli either directly or via memory retrieval. In a constantly changing environment, animals have evolved attention-like processes to effectively filter the incoming sensory stream. These attention-like processes, in turn, are modulated by memory. The neurobiological nature of how attention, action selection and memory are inter-connected is unknown. We describe here new phenotypes of the memory mutant radish in the fruit fly Drosophila.
Mechanisms of plasticity in simple taxis behaviors in Drosophila
Nov 8
Like the proverbial moth drawn to the candle flame, the fruit fly Drosophila also stereotypically approaches light sources. This positive phototaxis is the archetypal example of hard-wired input-output behaviors.
Live cell imaging of mutant and wild-type GABA-A receptor trafficking using a novel reporter protein
Efficient signaling in the brain requires precise regulation and targeting of cell surface ion-channels. Mutations in these channels associated with inherited diseases can cause improper targeting and reduced surface expression.
ADP-Glo™: An Ideal Approach to Monitor the Activity of Protein Kinases and Beyond
Oct 16
Because of its versatility (alltypesofsubstrates), robustness (Z’>0.8), rapidperformance, and its ease of use, the luminescence based Kinase Glo® assay platform has gained wide acceptance in many drug screening programs for protein kinase inhibitors.
more POSTERS >>
Crosstalk between planar cell polarity signaling and miR-8 control of NHERF1-mediated actin reorganization.
5 hours ago
The response to osmotic stress in developing zebrafish embryos requires proper apical patterning and trafficking of transmembrane ion transporters in ionocytes, specialized cells of the epidermis. The miR-8 family of miRNAs plays a key role in this process by...
A systematic approach to understand the functional consequences of non-protein coding risk regions.
A primary goal of genetic association studies is to elucidate genes and novel biological mechanisms involved in disease. Recently, genome-wide association studies have identified many common genetic variants that are significantly associated with complex...
A role for Chk2 in DNA damage induced mitotic delays in human colorectal cancer cells.
7 hours ago
Progression into mitosis in the presence of DNA damage leads to spindle checkpoint (SAC) dependent mitotic delays and cytokinesis failure. In Drosophila embryos, DNA damage does not delay mitotic entry but triggers Checkpoint kinase-2 (Chk2) kinase dependent...
An ex vivo swine tracheal organ culture for the study of influenza infection.
8 hours ago
Please cite this paper as: Nunes et al. (2009) An ex vivo swine tracheal organ culture for the study of influenza infection. Influenza and Other Respiratory Viruses 4(1), 7-15.Background The threat posed by swine influenza viruses with potential to transmit from...
more JOURNAL ARTICLES >>
Prokariotic Cell Collection in Denmark
I would like to know about a prokariotic cell collection in Denmark. Is there a cell bank in this country? I need a Lactobacillus strain for a fermentation assay and this information about the bank is very helpful for me.
Request for Entries
Ask the Experts is your chance to get the answers to questions on applications, materials, methods, processes, and technologies. Email you question to bst_web@advantagemedia.com, and the editors of Bioscience Technology will find an appropriate expert to answer it. Watch this space in the future to see the questions your colleagues are posting.
more ASK THE EXPERTS >>
Social services survive Conn. budget axe _ for now
1 hour ago
Medicines Co. buys Pfizer heart drug candidate
2 hours ago
Enzon shareholders to vote on sale on Jan. 27
3 hours ago
West Pharmaceutical hikes dividend by a penny
4 hours ago
Keep IRS auditors away: earn less than $200,000
Chemistry makes the natural 'wonder fabric' -- wool -- more wonderful
Climate debate: What's warming us up? Human activity or Mother Nature?
more NEWS >>
Dr. Robert Langer Joins Millipore’s Board of Directors
Dec 18 | News
Beckman Coulter, Inc. to Distribute CompuCyte Laser Scanning Cytometers
Dec 11 | News
Paul Chapman, Millipore Vice President, Downstream Processing Presents on Single-Use Solutions at 2009 BIO International Convention
Dec 8 | News
Dec 2 | White Papers
OET Signs Two New Research Collaboration Agreements
Dec 2 | News
more SUPPLIER UPDATES >>
Sample Preparation Reagent Provides Reverse Transcription-ready RNA
Nov 20
Depression, traumatic stress and interleukin-6.
Dec 18
X-ray absorption spectroscopy of biomimetic dye molecules for solar cells.
Nov 26
Adaptation and transmission of a duck-origin avian influenza virus in poultry species.
Dec 11
5 hours ago | PubGet
ATM germline mutations in women with familial breast cancer and a relative with haematological malignancy.
10 hours ago | PubGet
Coronary artery bypass graft surgery depletes plasma thiamine levels.
Dec 21 | PubGet
The synergistic antimicrobial effect by mechanical agitation and two chlorhexidine preparations on biofilm bacteria.
Bupivacaine 0.25% and methylene blue spread with epidural anesthesia in dog.
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Neuroscience Diseases of The Brain and How The Mind Emerges
Dennis Choi, director of Emory Universitys Neuroscience Center, is renowned for his groundbreaking research on brain and spinal cord injury.
more VIDEO >>
Allen Institute for Brain Research
Oct 14
Discussed in this interview are both the mouse brain project and the human cortex project with an emphasis on the importance of these projects to neuroscience research.
more PODCASTS >>