Double-Stranded RNA Fragment Creation

RNase III, derived from E. coli, is an endoribonuclease that digests double-stranded RNA (dsRNA) into short, dsRNA fragments with 2-base, 3 feet-overhangs. The enzyme has been shown to be an efficient and effective method for generating a heterogeneous population of short dsRNA from a long in vitro transcript. Proprietary RNase III preparations are active against dsRNA and purified free of contaminating endo- and exonucleases.