Glutathione Reductase Assay Kit

High volume assay kit measures cellular levels of glutathione reductase, an enzyme that catalyzes the reduction of oxidized glutathione (GSSG) to reduced glutathione (GSH). GSH is an important intracellular antioxidant that reacts with oxygen free radicals and organic peroxides and acts as a substrate for the other detoxification enzymes such as glutathione peroxidase and glutathione S-transferase. The activity of glutathione reductase is an important measure of the antioxidant status of the cell. Glutathione reductase requires NADPH for its activity, resulting in the reduction of GSSG to GSH and the corresponding oxidation of NADPH to NADP. The assay takes less than 6 minutes to perform and is based on the measurement of the loss in absorbance at 340 nm due to the conversion of NADPH to NADP. The kit contains everything needed to perform the assay on 100 samples and its corresponding blanks, each in duplicate, as well as 10 standard curves with each point performed in duplicate.