Nitrotyrosine Assay, Antibody

Nitrotyrosine has been identified as a marker of inflammation and NO production. Nitrotyrosine is formed in presence of the active metabolite NO. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production. Since nitrotyrosine is a stable end product of peroxynitrite oxidation, assessment of its plasma concentration may be useful as a marker of NO-dependent damage in vivo. Since NO_X is only an indicator for enhanced NO production, protein associated nitrotyrosine might be a more suitable marker for damage induced by reactive nitrogen intermediates derived from NO. Furthermore, most proteins have a longer half-life in the circulation than NO_X levels. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic placques, celiac disease, rheumatoid arthritis, chronic renal failure and septic shock. HyCult Biotechnology b.v. has developed a sensitive ELISA for detection of Nitrotyrosine as well as an excellent monoclonal antibody against Nitrotyrosine for Western blot and immunohistochemistry of frozen and paraffin sections.