Rapid Affinity Purification Kits

RAP™ (Rapid Affinity Purification) Kit is a complete kit for making antigen specific solid matrix for use in affinity purification. The kit provides a ready-to-use coupling gel for covalent immobilization of sulfhydryl-containing compounds, such as peptides and proteins. The coupling gel is prepacked in a column and contains a 14-atom spacer arm with free terminal maleimide group that spontaneously reacts with the sulfhydryl group of cysteine in peptides or proteins to form stable C-S bond. After the immobilization with peptides or proteins, the affinity matrix is ready to purify anti-peptide (protein) antibodies.