Protein Discovery Chemistry

ProteoSep® protein discovery chemistry and software platform is offered through the Eprogen Protein Discovery Laboratory in kit format. ProteoSep all-liquid phase protein mapping technology uses standard HPLC instrument technology to produce high-resolution 1D and 2D maps of complex protein systems and serves as an alternative to 2D PAGE. Proteins are separated in the first dimension based on pI information using a high performance chromatofocusing (CF) column. Subsequent analysis of these pI fractions with Eprogen's proprietary reverse phase NPS® columns provides for the second dimension separation information based on hydrophobicity. A "2D protein map" is produced using Eprogen's ProteoSep Software Suite, displaying the proteins in bands like that presented in a 2D PAGE gel. IPAC® resins allow efficient separation of target proteins of interest, such as phosphorylated, glyco-, calcium-binding, and histidine proteins, quickly and with a high degree of accuracy.