Bacterial Protein Extraction Reagent (9497)

SoluLyse bacterial protein extraction reagent provides an efficient method for bacterial cell lysis and protein extraction under non-denaturing conditions. The reagent is quite compatible with purification resins, resulting in high yield of purified proteins. SoluLyse is a simple solution containing a proprietary nonionic detergent that is capable of cell wall perforation without denaturing proteins. With this gentle treatment, soluble proteins remain soluble and are ready to be fully extracted. In addition, viscosity from the release of DNA and RNA is minimized. As a result, cell lysates are easy to work with, and there is no need to add nucleases. The reagent is tested to be compatible with all popular purification methods, such as Ni and glutathione resins for purifying poly-His and GST-tagged proteins. To use, simply add the reagent to a bacterial pellet and gently pipette up and down, and incubate for 10 minutes. The protein of interest will be safely released into a cleared lysate for the next experiment. The reagnet leaves inclusion bodies are left intact in the cell debris and can be used to purify the inclusion bodies. If a detergent-free buffer condition is desired, SoluLyse may be readily dialyzed. Finally, additives such as protease inhibitors and reducing agents may be added in the presence of the reagent.