Cellular HDAC Assay Kit

The HDAC Fluorimetric Cellular Activity Assay provides the reagents and protocols needed for determining rates of intracellular deacetylase activity within cultured cells and is suitable for high-throughput cell-based deacetylase assays. HDACs are typically found in multi-protein complexes and are tightly regulated by subcellular localization, phosphorylation, and likely by other mechanisms. This assay allows the determination of deacetylase activity and provides accurate information that is reflective of endogenous regulation. It also allows the study of the effects of upstream regulators on deacetylase activity and the detection of inhibitors or activators that act indirectly to affect deacetylase activity.