Transfection Of Sensitive Cells
PromoFectin provides reliable delivery of nucleic acids into a variety of cell types, including many hard-to-transfect cell lines and primary cells. It consists of a robust, proprietary non-liposomal polymer that is stable, water-soluble and free of any components of animal origin. Low residual toxicity makes it useful for transfection of sensitive cells. Transfection with PromoFectin requires no medium change and it can be used for delivery of plasmid DNA as well as oligonucleotides and siRNA into adherent and non-adherent cells. It compacts and protects the nucleic acid of interest and endocytosed PromoFectin/Nucleic acid complexes enter the cell efficiently, rapidly and almost completely releasing intact nucleic acids into the cytosol. PromoFectin also favors DNA trafficking to the cell nucleus.
