Collagen ELISA Kit

Collagen IV full-sandwich ELISA kit includes a plate that has been coated with a Collagen IV capture reagent and blocked to reduce non-specific binding. The user adds the standard curve (provided) and samples to the plate and, following an incubation and wash, adds detection reagent labeled with HRP. After an additional incubation and plate wash, TMB substrate is added to the plate and the colorimetric reaction is stopped by the addition of 1N sulfuric acid. Absorbance at 450 nm is measured and the concentration of samples determined by comparison to the standard curve.