Mutation Detection Kits (9793)

High-throughput SURVEYOR Mutation Detection Kit for universal primer fluorescent capillary electrophoresis digests target DNA amplified with two universal fluorescent primers (included in the kit), labeled with either fluorescein or TAMRA, for subsequent analysis by fluorescent capillary electrophoresis using an Applied Biosystems PRISM Genetic Analyzer. SURVEYOR nuclease, a mismatch-specific endonuclease, recognizes mismatches in heteroduplex dsDNA and cleaves both DNA strands on the 3’-side of the mismatch site. It recognizes all base-substitutions, insertions, deletions and SNP’s, including multiple mutations, in single fragments, low copy mutations and pooled DNA samples.