TIRF Microscope Illuminator

Multi-color, multi-angle illuminator enables simultaneous multi-channel laser total internal reflectance fluorescence (TIRF) illumination. It allows up to three discrete laser lines to be used simultaneously for illumination, each with its own critical angle. In TIRF, the light’s angle of incidence dictates how deeply the evanescent wave penetrates into tissue, and lasers of different wavelengths require varying angles to operate at their peak.