Particle Characterization System Detects Proteins

The chromatography flow-mode option for the Malvern Instruments Zetasizer Nano particle and molecular characterization system enables its use as an in-line chromatography detector for protein applications. This option permits the coupling of dynamic light scattering with size exclusion chromatography, conferring the ability to perform Absolute Size Exclusion Chromatography (ASEC). This allows protein size to be measured directly rather than relying on column calibration or additional data from a second detector, and means that molecular weight standards are not required to identify oligomeric species or determine protein purity.