PCR Cloning Technology

GC Cloning is analogous to TA cloning and offers advantages over TOPO cloning, including many more recombinants, the ability to clone nanogram amounts of DNA, no colony screening, a universal format for cloning PCR products from both non-proofreading and proofreading DNA polymerases with the same kit. New kits contain pre-processed ready-to-ligate vector, enzymes and reagents for both PCR and ligation, and high efficiency competent cells.