MiRNA Labeling And Detection Kit

miRtect-IT miRNA labeling and detection kit detects miRNA in as little as 50 ng or less of total RNA. The kit’s linear detection range is 0.1 -10 femtomoles, and mature miRNA can be measured without an amplification step. One labeling reaction is used for the detection of any miRNA of interest, and a variety of small RNAs can be detected. The kit uses splinted-ligation technology, a nucleic acid hybridization assay that uses a miRNA-specific bridge oligonucleotide to form base pairs with the miRNA and a detection oligonucleotide. Captured miRNA is subsequently ligated to the detection oligonucleotide with T4 DNA Ligase. By joining the miRNA to the radiolabeled detection oligonucleotide, the assay directly labels miRNAs and avoids the inherent difficulty of detecting small RNAs of 20 – 24 nucleotides in Northern blots. Quantitative measurement of miRNAs in total RNA samples can be completed within 6 hr.