Mycoplasma Detection, Elimination

LookOut Mycoplasma PCR Detection Kit (Sigma-Aldrich, St. Louis, MO) includes pre-coated reaction vials that reduce prep time. The kit is optimized for use with JumpStart Taq DNA Polymerase (Sigma-Aldrich), and enables cell culture supernatants to be tested directly and without prior preparation. Nineteen species of mycoplasma can be detected (the kit is not suitable for detection of clinically associated species). Proprietary cocktails that eliminate mycoplasma from cultures are also available. Mammalian and viral cultures are mycoplasma-free in 3 passages (not suitable for Chlamydiacultures). The products are gentle on cells, with minimal cytotoxicity and are made of stable components (storage at ambient temperature,effective at 37 C). They are suitable for the elimination of mollicutes and related organisms (mycoplasma, acholeplasma, spiroplasma,and entomoplasma).