Fluorescent Acrylamide Solution

NEXT GEL ready-to-pour acrylamide solution contains a fluorescent protein stain for rapid band visualization following SDS-PAGE. The matrix eliminates the need for stacking gels and provides very high band resolution over a broad molecular weight range. The product is supplied as a 1× acrylamide solution that requires only APS and TEMED for polymerization, and comes complete with Running Buffer. It incorporates an in-gel stain that replaces post-run fixing, staining and destaining steps. After electrophoresis the gel is placed on a UV Transilluminator where protein bands are visible within three to five minutes. The proprietary probe covalently cross-links to the protein upon exposure to UV irradiation and then emits a bright, white light. Sensitivity is comparable to staining with Coomassie Brilliant Blue R-250.