Protein Quantitation Kits (10871)

Pierce SILAC (Stable Isotope Labeling with Amino Acids in Cell Culture) Protein Quantitation Kits provide comprehensive identification, characterization and quantitation of proteins. Isotopic labeling of the proteome for the analysis of relative protein abundance by mass spectrometry (MS) provides a complete solution. In vivo labeling incorporates M¹³C- or ¹⁵N-labeled amino acids directly into proteins using normal cellular metabolism of living cells. This strategy provides 100% label incorporation into proteins prior to digestion. The kits include HyClone media, heavy and light amino acids, and HyClone Dialyzed Serum in a single package. Protein expression levels can be quantitated between differentially treated cell populations. This is made possible because the heavy and light amino acids are chemically identical to each other, yet isotopically distinct. By employing high resolution analysis with the Thermo Scientific LTQ-FT Ultra and LTQ Orbitrap, the differentially expressed proteins are detected by MS analysis based on relative peak intensity.