Detect DNA Damage

DNA damage caused by oxidative radicals is associated with aging, inflammation, carcinogenesis, Parkinson’s and Alzheimer’s diseases. When exposed to oxidative radicals, 8-hydroxy-2’-deoxyguanosine (8-oxo-dG) is formed and can serve as a sensitive indicator of physiological and environmental damage to DNA. This mouse monoclonal antibody specifically binds to 8-hydroxy-2’- deoxyguanosine within DNA in H2O2-treated cells. It can be used to detect oxidative damage by ELISA and immunocytochemistry. Sufficient antibody is provided for approximately 50 slides, when a 1:250 dilution is used.

H2O2 treated (A) and untreated (B) MCF-10A cells stained with 8-oxo-dG antibody according to the above protocol using AlexaFluor 488 conjugated anti-mouse antibody.