DNA Dephosphorylation And Ligation Kit

Rapid DNA Dephos & Ligation Kit provides dephosphorylation and ligation of sticky- or blunt-end DNA fragments, enabling ligation of DNA in 5 minutes and dephosphorylation in 10 minutes. The kit contains the new recombinant rAPid Alkaline Phosphatase, which is easily heat-inactivated in 2 minutes at 75 C, eliminating phenol-chloroform extraction steps and allowing dephosphorylation to be performed directly in restriction enzyme digests. Procedures are thus simplified for a broad array of applications, including cloning of fragments into plasmid or phage vectors, linker ligation, recircularization of linear vector DNA, and generation of libraries.