Product Releases
Protease Mix Enhances Proteolysis of Difficult-to-Digest Proteins
Tue, 2024-03-05 12:29
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Promega Corporation announced the launch of Trypsin/Lys-C Mix, Mass Spectrometry Grade. A combination of trypsin gold and rLys-C proteases, the new mix increases peptide generation with in-solution protein digestion and works with even difficult to digest proteins. Enhanced proteolytic activity achieved through use the mix can result in a 20 to 40 percent increase in the number of peptides generated, enabling a higher number of proteins to be identified. The mix is also tolerant to trypsin-inhibiting contaminants, enabling researchers to generate mass spectrometry data from poor-quality sample material such as FFPE sections. Many proteins are resistant to trypsin due to tight conformation, and although protein denaturation enables proteolysis, trypsin is inactive under these conditions. The mix overcomes this barrier as it remains active even under strong denaturing conditions, enabling digestion of even proteolytically resistant proteins.
Promega Corporation, 608-274-4330, www.promega.com/tryplys
