Image Analysis via Mobile Devices for Life Science Research
Leica Microsystems offers the Leica Application Suite Advanced Fluorescence (LAS AF), a software platform for advanced life science research in widefield and confocal microscopy, in its 4.0 version. With LAS AF researchers can now perform 2D image analysis on multi-channel experiments and fully control the environmental conditions of an experiment. In addition, users of widefield systems can now connect to the acquisition station remotely anywhere at any time.
A workflow guides users step by step through the 2D image analysis from applying filters, thresholding, and binary image processing, to measurements and classification. Results are obtained easily, quickly, and reproducibly even with multi-dimensional data sets. Each analysis step offers a set of tools that researchers can choose according to the analysis requirements. The image viewer provides immediate feedback on the applied settings. Analysis workflows can be saved as user-defined protocols and applied to further data sets at a later time. The analysis results can be saved with the experiment or exported as Excel reports together with histograms and images of intermediate steps for documentation or further analysis. For statistical purposes users can analyze several data sets of experiments in one run by simply adding the data to a batch list.
Samples with several fluorescent markers can be completely analyzed in one run by using separate analysis channels for different markers. Optionally, objects identified in different acquisition channels can be correlated, for example to count the number of DNA strand breaks per nucleus. 2D analysis can also be applied to color images – for example to determine the number of healthy cells in one analysis channel versus the number of abnormal cells in another analysis channel.
Mobile Connection works via web client or mobile device. As several users can be connected with the microscope system at the same time, they can also interact with each other, exchanging messages to discuss the most recent developments of a live experiment.
Leica Microsystems, 800-248-0123, www.leica-microsystems.com
