Reagent Delivers qPCR Data from Challenging Samples
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Bio-Rad Laboratories launched the SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix, a solution for obtaining reproducible, optimal-quality qPCR data from the most challenging samples. Generating quality qPCR data from difficult-to-amplify samples such as crude lysates from plants, tissues, formalin-fixed paraffin-embedded samples, and other less-than-ideal sources that contain PCR inhibitors can be challenging and frustrating. In these situations, PCR may be greatly inhibited, making it nearly impossible for researchers to obtain reliable data, wasting time and money.
The formulation of the SsoAdvanced Universal Inhibitor-Tolerant SYBR Green Supermix has been optimized and validated to tolerate a wider spectrum of PCR inhibitors than competing SYBR Green reagents. These inhibitors include those found in crude lysates, polysaccharides and polyphenols, and various reagents left over from sample prep such as ethanol, isopropanol, EDTA, and sodium chloride (see performance data for all inhibitors).
As with all SsoAdvanced Supermixes, the inhibitor-tolerant version includes Bio-Rad’s Sso7d-fusion polymerase, which is engineered for enhanced qPCR performance. The supermix is compatible with all real-time PCR instruments and functions under any reaction condition.
Bio-Rad Laboratories, Inc., 800-424-6723, www.bio-rad.com/SUIT1
