Digital PCR System Enables Accurate Diagnostic Detection

Medical practitioners in Europe can use Bio-Rad’s CE-IVD marked QX200 Droplet Digital PCR (ddPCR) System, IVD for highly-accurate diagnostic detection and quantification of nucleic acids, aiding clinical decision-making in the treatment of diseases ranging from cancer to transplant rejection and viral infection.

The system includes a QX200 Droplet Generator, QX200 Droplet Reader, and QuantaSoft software. The droplet generator and reader are CE-IVD marked for use in the European Union for in vitro diagnostics and are the only digital PCR instruments available for use in a clinical laboratory.

The generator is used to partition a ddPCR reaction mixture into thousands of nanoliter-sized droplets. After endpoint PCR on a thermal cycler, droplets are read individually as they are streamed single file past a two-color optical detection system on the droplet reader. The PCR-positive and PCR-negative droplets are counted to provide absolute quantification of target DNA.


The Digital PCR System, IVD features:
• Most precise and sensitive digital PCR solution for a wide variety of diagnostic applications
• Flexible digital PCR chemistry, optimized for TaqMan probe and EvaGreen applications
• Convenient assay design – no standard curves
• Flexible assay setup – scalable for high sensitivity or high throughput
• Easy-to-use workflow with 96-sample throughput

Applications:
• Liquid biopsy cancer detection and monitoring
• Gene editing validation
• Viral load and residual disease monitoring
• Copy number determination
• Gene expression analysis

Bio-Rad Laboratories Inc. 1-800-224-6723, www.bio-rad.com