BREA, Calif. (September 16, 2024) – Beckman Coulter,
Inc. introduces Krome Orange, a novel violet-excited organic dye
that expands the range of available choices and dye sensitivity
limits for violet lasers. Common gating markers can be easily
transferred to this fluorochrome, freeing other channels for use
with more esoteric markers and enabling more versatile 10-color
flow cytometry applications.
Krome Orange has excitation and emission maxima of 398 nm and
528 nm respectively and provides a brighter signal than Pacific
Orange* conjugates with equivalent spectral overlaps into adjacent
channels. The new dye is at least as bright as V500 and can provide
more than twice the population separation obtained with Pacific
Orange dye conjugates, with little compensation compared to Pacific
Blue* dye.
Krome Orange conjugates show optimal performance with violet
laser excitation and a 550/40 bandpass filter (the standard FL10
channel on flow cytometry systems), while no excitation is detected
using a 488 nm laser. The initial release of Krome Orange includes
conjugates to human CD45 and CD4, with the range of human and mouse
targets to expand going forward.
"Krome Orange provides scientists with the ability to increase
sensitivity for low fluorescence signals and to expand their
flexibility for multi-color flow cytometry experiments," said Brad
Calvin, vice president and general manager of the Beckman Coulter
Flow Cytometry Business Center. "The development of this new dye
underscores Beckman Coulter's commitment to expand multi-color
cellular analysis technologies."
About Beckman Coulter
Beckman Coulter, Inc., based in Orange County, California,
develops, manufactures and markets products that simplify, automate
and innovate complex biomedical tests. This year marks the
company's 75th anniversary of advancing healthcare. More than
200,000 Beckman Coulter systems operate in laboratories around the
world, supplying critical information for improving patient health
and reducing the cost of care. Recurring revenue, consisting of
consumable supplies (including reagent test kits), service and
operating-type lease payments, represents about 80 percent of the
company's 2009 revenue of $3.3 billion. For more information,
visit www.beckmancoulter.com or www.beckmancoulter.com/75years.
Photo Caption: Krome Orange dye excitation curve (dashed black
line); emission curves for Krome Orange dye, Pacific Orange dye,
AmCyan, and V500 dye. The shaded region represents the standard
550/40 band pass filter installed into FL10 on a cytometer.
*Pacific Orange and Pacific Blue are trademarks of Life
Technologies Corp. Beckman Coulter and the stylized logo are
registered trademarks of Beckman Coulter, Inc. Krome Orange is a
trademark of Beckman Coulter, Inc. Analyte Specific Reagent:
Analytical and performance characteristics are not established.
Product regulatory status varies dependant on the country where the
product is distributed.
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