Collagen Antibody Kit Induces Arthritis in 24 Hours
Fri, 2024-03-01 14:43

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AMSBIO announced a new 5-Clone Collagen Antibody-Induced Arthritis (CIAA) kit for the induction of Collagen-Induced Arthritis (CIA) in various species. Collagen Antibody-Induced Arthritis (CAIA) has been widely used since 1998 when 4-clone cocktail (Arthrogen-CIA arthritogenic monoclonal antibodies) kits first appeared. In order to induce consistent arthritis more effectively in various mouse strains, including CAIA low responder C57BL/6 mice, and a variety of unpredictable knockout and transgenic mice with C57BL/6 (H-2b) or 129/Sv (H-2b) backgrounds, a new 5-clone cocktail kits have been developed by adding a new clone to the 4-clone cocktail. The new kit produces faster results: Typically severe arthritis is induced within 24–48 hours instead of the four weeks required to induce CIA after immunization with type II collagen. Unlike the original CIA model, which requires a restricted MHC haplotype, the new kit is broadly applicable and can induce arthritis in almost all strains of mice, including CIA-resistant, T-cell deficient, gene knockout and transgenic mice. Beneficially, the new kits offer excellent consistency, meaning the severity of arthritis induced now directly correlates to the dose of monoclonal antibody cocktail. More severe and consistent arthritis can be induced by increasing the dose of monoclonal antibody cocktail. The kits are ideal for studying the pathological role of individual gene products, cytokines, as well as screening and evaluating anti-inflammatory agents without influences of complete or incomplete Freund's adjuvant, which strongly affects the host's immune system.
 

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