CESI-MS System for Therapeutic Biologics

The CESI 8000 System for Biologics Characterization from AB Sciex, a separation-electrospray ionization system for mass spectrometry (MS), combines of capillary electrophoresis (CE) and electrospray ionization (ESI) technologies to create an integrated workflow solution. This front-end CESI technology from the company's SCIEX Separations business can be integrated with the AB Sciex TripleTOF 5600 mass spectrometry system into one solution, for example, to give biopharmaceutical scientists the ability to advance therapeutics discovery, research and development through the life cycle of the product.

With a single approach that increases efficiency and productivity, the CESI 8000 system provides orthogonal and/or superior results compared to dual-method liquid chromatography / mass spectrometry-based approaches.  From a single digest and run, users of CESI-MS gain the following: (a) Identity and Purity information with 100 percent coverage in a peptide map; (b) Heterogeneity information with ultrasensitive glycopeptide quantitation; and (c) Stability information identifying, deamidation, cyclization and oxidation.

The technical benefit of the CESI innovation delivered to biopharmaceutical laboratories is an improvement in biophysicochemical characterization to guide the development of biologics-based therapeutics.  Specifically, CESI-MS serves to improve ionization efficiency and reduce ion suppression.  This advancement offers a robust solution without requiring specialized expertise in CE or MS.

Switching between LC-MS and CESI-MS has also been made simple, which will help facilitate adoption of CESI-MS technology.  The data that is generated on the CESI-based workflow is complementary to data from LC-MS/MS solutions.

AB Sciex, www.sciex.com/cesi