TripleTOF System for Quantitative Proteomics
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AB SCIEX introduced the TripleTOF 6600 system with SWATH Acquisition 2.0—the company’s solution for quantitative proteomics. This high-resolution, accurate-mass system, combined with the new SWATH Acquisition 2.0, brings new power to proteomic data challenges for advanced biomarker discovery and systems biology. As the emphasis in proteomics research shifts from cataloguing to comprehensive quantitation, the AB SCIEX solution enables the quantification of thousands of proteins across large sample sets with a high level of data completeness and quantitative accuracy and precision.
AB SCIEX SWATH Acquisition incorporates a data-independent acquisition strategy and fragments every detectable peptide in the sample in order to acquire quantitative MS/MS data. This approach has been shown to increase the capacity of targeted proteomics experiments by over 30-fold and reduce study time by as much as 90 percent. Now, with the increased dynamic range of the TripleTOF 6600 System, researchers can more deeply interrogate complex samples. Combined with variable window acquisition and the SWATH 2.0 processing software, a significant improvement in quantitative results has been realized.
Early experiments comparing TripleTOF 6600 System with SWATH 2.0 to other systems have demonstrated the quantification of up to 90 percent more peptides with CVs of less than 20 percent (a threshold often used to determine good limits of quantitation in LC/MS data). These advances in capacity and data completeness are expected to make integration with large-scale biology experiments, such as genomics and transcriptomics, much more accessible.
AB SCIEX, 877-740-2129, www.absciex.com
