PrimePCR Assays

Primer design is a crucial, but time-consuming and laborious first step for gene expression analysis with real-time PCR. Guaranteed to perform, Bio-Rad’s PrimePCR Assays remove this bottleneck. Bio-Rad is introducing expertly designed PrimePCR gene expression assays for nine new genomes that were designed using the same algorithm that was thoroughly optimized during the wet-lab validation process for human, mouse, and rat gene expression assays. “We tested almost 100,000 assays, optimizing our design algorithm with every gene expression assay that did not meet stringent performance criteria,” remarks Brandon Selby, Product Manager in Bio-Rad’s Gene Expression Group. By using the assays, researchers can rest assured that they are following industry best practices known as MIQE (minimum information for publication of quantitative real-time PCR experiments). The gene expression assays are available in SYBR Green- and probe-based formats for a wide range of targets, as PreAmp assays for preamplification, and as control assays and templates.

Bio-Rad Laboratories Inc., www.bio-rad.com