Multiplex Detection of Oxidative Phosphorylation (OXPHOS)

Mitochondria are unique and complex organelles that perform essential functions in many aspects of cell biology. The dominant function of mitochondria is the production of more than 90% of the cell’s energy in the form of ATP through oxidative phosphorylation. Mitochondrial dysfunction is associated with multiple diseases including cancer, diabetes, neurodegenerative disease, as well as aging. Studies have revealed that the toxic effects of certain drugs are due mitochondrial dysfunction. We describe a method for multiplexed analysis of OXPHOS from cell lysate and tissue extract using the MILLIPLEX® MAP Human Oxidative Phosphorylation and the MILLIPLEX® MAP Rat/Mouse Oxidative Phosphorylation panels.